Comparative genomic hybridization (CGH) is a molecular cytogenetic method for analysing copy number variations (CNVs) relative to ploidy level in the DNA of a test sample compared to a reference sample, without the need for culturing cells. (http://en.wikipedia.org/wiki/Comparative_genomic_hybridization)
This technique was originally developed for the evaluation of the differences between the chromosomal complements of Solid tumor and normal tissue, and has an improved resolution of 5-10 megabases compared to the more traditional cytogenetic analysis techniques of giemsa banding and fluorescence in situ hybridization (FISH) which are limited by the resolution of the microscope utilized.
- Conventional CGH
- Array CGH